LOCUS       40924_23594       12218 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Gateway binary vector pGWB655 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12218)
  AUTHORS   Nakamura S, Mano S, Tanaka Y, Ohnishi M, Nakamori C, Araki M, Niwa 
            T, Nishimura M, Kaminaka H, Nakagawa T, Sato Y, Ishiguro S.
  TITLE     Gateway binary vectors with the bialaphos resistance gene, bar, as a
            selection marker for plant transformation
  JOURNAL   Biosci. Biotechnol. Biochem. 74 (6), 1315-1319 (2010)
  PUBMED    20530878
REFERENCE   2  (bases 1 to 12218)
  AUTHORS   Tanaka Y, Nakamura S, Ishiguro S, Nakagawa T.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JAN-2010) Contact:Tsuyoshi Nakagawa Shimane 
            University, Center for Integrated Research in Science; 1060 
            Nishikawatsu, Matsue, Shimane 690-8504, Japan
REFERENCE   3  (bases 1 to 12218)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12218)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci. 
            Biotechnol. Biochem."; date: "2010"; volume: "74"; issue: "6"; 
            pages: "1315-1319"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-JAN-2010) Contact:Tsuyoshi Nakagawa Shimane University, Center 
            for Integrated Research in Science; 1060 Nishikawatsu, Matsue, 
            Shimane 690-8504, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     constructed using pPZP221.
FEATURES             Location/Qualifiers
     source          1..12218
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(54..78)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             1178..1852
                     /codon_start=1
                     /label=mRFP1
                     /note="monomeric derivative of DsRed (Campbell et al.,
                     2002)"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     protein_bind    1862..1986
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        2023..2053
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             2107..2763
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             3108..3410
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(3454..3578)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      3604..3856
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3890..3906)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3914..3930
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3938..3968)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3983..4004)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     terminator      complement(4040..4292)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4455..5003)
                     /codon_start=1
                     /label=BlpR
                     /note="phosphinothricin acetyltransferase"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(5055..5234)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    complement(5740..5764)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6285..7073
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7322..7910
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(8096..8236)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8580..8774)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8843..9913)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10345..10971)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag aatgagcatg gcctcctccg aggacgtcat
     1201 caaggagttc atgcgcttca aggtgcgcat ggagggctcc gtgaacggcc acgagttcga
     1261 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt
     1321 gaccaagggc ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tccagtacgg
     1381 ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc
     1441 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac
     1501 ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa
     1561 cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg cctccaccga
     1621 gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagatgaggc tgaagctgaa
     1681 ggacggcggc cactacgacg ccgaggtcaa gaccacctac atggccaaga agcccgtgca
     1741 gctgcccggc gcctacaaga ccgacatcaa gctggacatc acctcccaca acgaggacta
     1801 caccatcgtg gaacagtacg agcgcgccga gggccgccac tccaccggcg ccgctgttat
     1861 cacaagtttg tacaaaaaag ctgaacgaga aacgtaaaat gatataaata tcaatatatt
     1921 aaattagatt ttgcataaaa aacagactac ataatactgt aaaacacaac atatccagtc
     1981 actatggcgg cccctcgagg ggccgcatta ggcaccccag gctttacact ttatgcttcc
     2041 ggctcgtata atgtgtggat tttgagttag gatccgtcga gattttcagg agctaaggaa
     2101 gctaaaatgg agaaaaaaat cactggatat accaccgttg atatatccca atggcatcgt
     2161 aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta cctataacca gaccgttcag
     2221 ctggatatta cggccttttt aaagaccgta aagaaaaata agcacaagtt ttatccggcc
     2281 tttattcaca ttcttgcccg cctgatgaat gctcatccgg aattccgtat ggcaatgaaa
     2341 gacggtgagc tggtgatatg ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa
     2401 actgaaacgt tttcatcgct ctggagtgaa taccacgacg atttccggca gtttctacac
     2461 atatattcgc aagatgtggc gtgttacggt gaaaacctgg cctatttccc taaagggttt
     2521 attgagaata tgtttttcgt ctcagccaat ccctgggtga gtttcaccag ttttgattta
     2581 aacgtggcca atatggacaa cttcttcgcc cccgttttca ccatgggcaa atattatacg
     2641 caaggcgaca aggtgctgat gccgctggcg attcaggttc atcatgccgt ttgtgatggc
     2701 ttccatgtcg gcagaatgct taatgaatta caacagtact gcgatgagtg gcagggcggg
     2761 gcgtaaacgc gtggatccgg cttactaaaa gccagataac agtatgcgta tttgcgcgct
     2821 gatttttgcg gtataagaat atatactgat atgtataccc gaagtatgtc aaaaagaggt
     2881 atgctatgaa gcagcgtatt acagtgacag ttgacagcga cagctatcag ttgctcaagg
     2941 catatatgat gtcaatatct ccggtctggt aagcacaacc atgcagaatg aagcccgtcg
     3001 tctgcgtgcc gaacgctgga aagcggaaaa tcaggaaggg atggctgagg tcgcccggtt
     3061 tattgaaatg aacggctctt ttgctgacga gaacaggggc tggtgaaatg cagtttaagg
     3121 tttacaccta taaaagagag agccgttatc gtctgtttgt ggatgtacag agtgatatta
     3181 ttgacacgcc cgggcgacgg atggtgatcc ccctggccag tgcacgtctg ctgtcagata
     3241 aagtctcccg tgaactttac ccggtggtgc atatcgggga tgaaagctgg cgcatgatga
     3301 ccaccgatat ggccagtgtg ccggtctccg ttatcgggga agaagtggct gatctcagcc
     3361 accgcgaaaa tgacatcaaa aacgccatta acctgatgtt ctggggaata taaatgtcag
     3421 gctcccttat acacagccag tctgcaggtc gaccatagtg actggatatg ttgtgtttta
     3481 cagtattatg tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca
     3541 ttttacgttt ctcgttcagc tttcttgtac aaagtggtga taacctagag ctcgaatttc
     3601 cccgatcgtt caaacatttg gcaataaagt ttcttaagat tgaatcctgt tgccggtctt
     3661 gcgatgatta tcatataatt tctgttgaat tacgttaagc atgtaataat taacatgtaa
     3721 tgcatgacgt tatttatgag atgggttttt atgattagag tcccgcaatt atacatttaa
     3781 tacgcgatag aaaacaaaat atagcgcgca aactaggata aattatcgcg cgcggtgtca
     3841 tctatgttac tagatcggga attggttccg gaaccaattc gtaatcatgg tcatagctgt
     3901 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     3961 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaggctga attaggcgcg
     4021 cctatttcta ttaattcccg atctagtaac atagatgaca ccgcgcgcga taatttatcc
     4081 tagtttgcgc gctatatttt gttttctatc gcgtattaaa tgtataattg cgggactcta
     4141 atcataaaaa cccatctcat aaataacgtc atgcattaca tgttaattat tacatgctta
     4201 acgtaattca acagaaatta tatgataatc atcgcaagac cggcaacagg attcaatctt
     4261 aagaaacttt attgccaaat gtttgaacga tcggggaaat tcgagctggg gacgtcccca
     4321 gctcggtacc ccctcctggg cacggtcgta cgcgatccgc cgcggttcca cctcaccatt
     4381 gcgggtatgc cagttgagtt cgatgagttc ccgtaccagc tccaactcgg gcgatgccac
     4441 gcgcactccg ttcagatctc ggtgacgggc aggaccggac ggggcggtac cggcaggctg
     4501 aagtccagct gccagaaacc cacgtcatgc cagttcccgt gcttgaagcc ggccgcccgc
     4561 agcatgccgc ggggggcata tccgagcgcc tcgtgcatgc gcacgctcgg gtcgttgggc
     4621 agcccgatga cagcgaccac gctcttgaag ccctgtgcct ccagggactt cagcaggtgg
     4681 gtgtagagcg tggagcccag tcccgtccgc tggtggcggg gggagacgta cacggtcgac
     4741 tcggccgtcc agtcgtaggc gttgcgtgcc ttccaggggc ccgcgtaggc gatgccggcg
     4801 acctcgccgt ccacctcggc gacgagccag ggatagcgct cccgcagacg gacgaggtcg
     4861 tccgtccact cctgcggttc ctgcggctcg gtacggaagt tgaccgtgct tgtctcgatg
     4921 tagtggttga cgatggtgca gaccgccggc atgtccgcct cggtggcacg gcggatgtcg
     4981 gccgggcgtc gttctgggct catatcttat tgccccccgg gatcagatct ggattgagag
     5041 tgaatatgag actctaattg gataccgagg ggaatttatg gaacgtcagt ggagcatttt
     5101 tgacaagaaa tatttgctag ctgatagtga ccttaggcga cttttgaacg cgcaataatg
     5161 gtttctgacg tatgtgctta gctcattaaa ctccagaaac ccgcggctga gtggctcctt
     5221 caacgttgcg gttctgtcag ttccaaacgt aaaacggctt gtcccgcgtc atcggcgggg
     5281 gtcataacgt gactccctta attctccgct catgatcttg atcccctgcg ccatcagatc
     5341 cttggcggca agaaagccat ccagtttact ttgcagggct tcccaacctt accagagggc
     5401 gccccagctg gcaattccgg ttcgcttgct gtccataaaa ccgcccagtc tagctatcgc
     5461 catgtaagcc cactgcaagc tacctgcttt ctctttgcgc ttgcgttttc ccttgtccag
     5521 atagcccagt agctgacatt catccggggt cagcaccgtt tctgcggact ggctttctac
     5581 gtgttccgct tcctttagca gcccttgcgc cctgagtgct tgcggcagcg tgaagctgat
     5641 ccgtcgagat taatagaaat aaattcagcc taattcggcg ttaattcagt acattaaaaa
     5701 cgtccgcaat gtgttattaa gttgtctaag cgtcaatttg tttacaccac aatatatcct
     5761 gccaccagcc agccaacagc tccccgaccg gcagctcggc acaaaatcac cactcgatac
     5821 aggcagccca tcagtccggg acggcgtcag cgggagagcc gttgtaaggc ggcagacttt
     5881 gctcatgtta ccgatgctat tcggaagaac ggcaactaag ctgccgggtt tgaaacacgg
     5941 atgatctcgc ggagggtagc atgttgattg taacgatgac agagcgttgc tgcctgtgat
     6001 caattcgggc acgaacccag tggacataag cctgttcggt tcgtaagctg taatgcaagt
     6061 agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg
     6121 cagtggcggt tttcatggct tgttatgact gtttttttgg ggtacagtct atgcctcggg
     6181 catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg
     6241 atgttacgca gcagggcagt cgccctaaaa caaagttaaa catcatgggg gaagcggtga
     6301 tcgccgaagt atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac
     6361 cgacgttgct ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca
     6421 gtgatattga tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt
     6481 tgatcaacga ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg
     6541 tagaagtcac cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg
     6601 aactgcaatt tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca
     6661 cgatcgacat tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg
     6721 taggtccagc ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc
     6781 taaatgaaac cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg
     6841 tagtgcttac gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg
     6901 atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg
     6961 aagctagaca ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt
     7021 tggaagaatt tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc aaataatgtc
     7081 tagctagaaa ttcgttcaag ccgacgccgc ttcgcggcgc ggcttaactc aagcgttaga
     7141 tgcactaagc acataattgc tcacagccaa actatcaggt caagtctgct tttattattt
     7201 ttaagcgtgc ataataagcc ctacacaaat tgggagatat atcatgcatg accaaaatcc
     7261 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     7321 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     7381 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     7441 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     7501 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     7561 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     7621 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     7681 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     7741 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     7801 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     7861 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     7921 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     7981 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     8041 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga
     8101 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca
     8161 gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga
     8221 ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
     8281 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
     8341 gaggttttca ccgtcatcac cgaaacgcgc gaggcagggt gccttgatgt gggcgccggc
     8401 ggtcgagtgg cgacggcgcg gcttgtccgc gccctggtag attgcctggc cgtaggccag
     8461 ccatttttga gcggccagcg gccgcgatag gccgacgcga agcggcgggg cgtagggagc
     8521 gcagcgaccg aagggtaggc gctttttgca gctcttcggc tgtgcgctgg ccagacagtt
     8581 atgcacaggc caggcgggtt ttaagagttt taataagttt taaagagttt taggcggaaa
     8641 aatcgccttt tttctctttt atatcagtca cttacatgtg tgaccggttc ccaatgtacg
     8701 gctttgggtt cccaatgtac gggttccggt tcccaatgta cggctttggg ttcccaatgt
     8761 acgtgctatc cacaggaaag agaccttttc gacctttttc ccctgctagg gcaatttgcc
     8821 ctagcatctg ctccgtacat taggaaccgg cggatgcttc gccctcgatc aggttgcggt
     8881 agcgcatgac taggatcggg ccagcctgcc ccgcctcctc cttcaaatcg tactccggca
     8941 ggtcatttga cccgatcagc ttgcgcacgg tgaaacagaa cttcttgaac tctccggcgc
     9001 tgccactgcg ttcgtagatc gtcttgaaca accatctggc ttctgccttg cctgcggcgc
     9061 ggcgtgccag gcggtagaga aaacggccga tgccgggatc gatcaaaaag taatcggggt
     9121 gaaccgtcag cacgtccggg ttcttgcctt ctgtgatctc gcggtacatc caatcagcta
     9181 gctcgatctc gatgtactcc ggccgcccgg tttcgctctt tacgatcttg tagcggctaa
     9241 tcaaggcttc accctcggat accgtcacca ggcggccgtt cttggccttc ttcgtacgct
     9301 gcatggcaac gtgcgtggtg tttaaccgaa tgcaggtttc taccaggtcg tctttctgct
     9361 ttccgccatc ggctcgccgg cagaacttga gtacgtccgc aacgtgtgga cggaacacgc
     9421 ggccgggctt gtctcccttc ccttcccggt atcggttcat ggattcggtt agatgggaaa
     9481 ccgccatcag taccaggtcg taatcccaca cactggccat gccggccggc cctgcggaaa
     9541 cctctacgtg cccgtctgga agctcgtagc ggatcacctc gccagctcgt cggtcacgct
     9601 tcgacagacg gaaaacggcc acgtccatga tgctgcgact atcgcgggtg cccacgtcat
     9661 agagcatcgg aacgaaaaaa tctggttgct cgtcgccctt gggcggcttc ctaatcgacg
     9721 gcgcaccggc tgccggcggt tgccgggatt ctttgcggat tcgatcagcg gccgcttgcc
     9781 acgattcacc ggggcgtgct tctgcctcga tgcgttgccg ctgggcggcc tgcgcggcct
     9841 tcaacttctc caccaggtca tcacccagcg ccgcgccgat ttgtaccggg ccggatggtt
     9901 tgcgaccgct cacgccgatt cctcgggctt gggggttcca gtgccattgc agggccggca
     9961 gacaacccag ccgcttacgc ctggccaacc gcccgttcct ccacacatgg ggcattccac
    10021 ggcgtcggtg cctggttgtt cttgattttc catgccgcct cctttagccg ctaaaattca
    10081 tctactcatt tattcatttg ctcatttact ctggtagctg cgcgatgtat tcagatagca
    10141 gctcggtaat ggtcttgcct tggcgtaccg cgtacatctt cagcttggtg tgatcctccg
    10201 ccggcaactg aaagttgacc cgcttcatgg ctggcgtgtc tgccaggctg gccaacgttg
    10261 cagccttgct gctgcgtgcg ctcggacggc cggcacttag cgtgtttgtg cttttgctca
    10321 ttttctcttt acctcattaa ctcaaatgag ttttgattta atttcagcgg ccagcgcctg
    10381 gacctcgcgg gcagcgtcgc cctcgggttc tgattcaaga acggttgtgc cggcggcggc
    10441 agtgcctggg tagctcacgc gctgcgtgat acgggactca agaatgggca gctcgtaccc
    10501 ggccagcgcc tcggcaacct caccgccgat gcgcgtgcct ttgatcgccc gcgacacgac
    10561 aaaggccgct tgtagccttc catccgtgac ctcaatgcgc tgcttaacca gctccaccag
    10621 gtcggcggtg gcccatatgt cgtaagggct tggctgcacc ggaatcagca cgaagtcggc
    10681 tgccttgatc gcggacacag ccaagtccgc cgcctggggc gctccgtcga tcactacgaa
    10741 gtcgcgccgg ccgatggcct tcacgtcgcg gtcaatcgtc gggcggtcga tgccgacaac
    10801 ggttagcggt tgatcttccc gcacggccgc ccaatcgcgg gcactgccct ggggatcgga
    10861 atcgactaac agaacatcgg ccccggcgag ttgcagggcg cgggctagat gggttgcgat
    10921 ggtcgtcttg cctgacccgc ctttctggtt aagtacagcg ataaccttca tgcgttcccc
    10981 ttgcgtattt gtttatttac tcatcgcatc atatacgcag cgaccgcatg acgcaagctg
    11041 ttttactcaa atacacatca cctttttaga cggcggcgct cggtttcttc agcggccaag
    11101 ctggccggcc aggccgccag cttggcatca gacaaaccgg ccaggatttc atgcagccgc
    11161 acggttgaga cgtgcgcggg cggctcgaac acgtacccgg ccgcgatcat ctccgcctcg
    11221 atctcttcgg taatgaaaaa cggttcgtcc tggccgtcct ggtgcggttt catgcttgtt
    11281 cctcttggcg ttcattctcg gcggccgcca gggcgtcggc ctcggtcaat gcgtcctcac
    11341 ggaaggcacc gcgccgcctg gcctcggtgg gcgtcacttc ctcgctgcgc tcaagtgcgc
    11401 ggtacagggt cgagcgatgc acgccaagca gtgcagccgc ctctttcacg gtgcggcctt
    11461 cctggtcgat cagctcgcgg gcgtgcgcga tctgtgccgg ggtgagggta gggcgggggc
    11521 caaacttcac gcctcgggcc ttggcggcct cgcgcccgct ccgggtgcgg tcgatgatta
    11581 gggaacgctc gaactcggca atgccggcga acacggtcaa caccatgcgg ccggccggcg
    11641 tggtggtgtc ggcccacggc tctgccaggc tacgcaggcc cgcgccggcc tcctggatgc
    11701 gctcggcaat gtccagtagg tcgcgggtgc tgcgggccag gcggtctagc ctggtcactg
    11761 tcacaacgtc gccagggcgt aggtggtcaa gcatcctggc cagctccggg cggtcgcgcc
    11821 tggtgccggt gatcttctcg gaaaacagct tggtgcagcc ggccgcgtgc agttcggccc
    11881 gttggttggt caagtcctgg tcgtcggtgc tgacgcgggc atagcccagc aggccagcgg
    11941 cggcgctctt gttcatggcg taatgtctcc ggttctagtc gcaagtattc tactttatgc
    12001 gactaaaaca cgcgacaaga aaacgccagg aaaagggcag ggcggcagcc tgtcgcgtaa
    12061 cttaggactt gtgcgacatg tcgttttcag aagacggctg cactgaacgt cagaagccga
    12121 ctgcactata gcagcggagg ggttggatca aagtactttg atcccgaggg gaaccctgtg
    12181 gttggcatgc acatacaaat ggacgaacgg ataaacct
//
